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This user manual provides operating instructions for the PBS-MINI Bioreactor platform and its 0.1 L and 0.5 L single-use Vessels. It covers system components, safety, installation, operation, maintenance, troubleshooting, and handling procedures. The manual explains how the Vertical-Wheel® Impeller and reusable base unit function together to support cell culture applications in a low-shear mixing environment. Technical specifications, sampling guidance, cleaning recommendations, and operating best practices support consistent performance in early-stage process development and research…

This user manual provides instructions for setting up, calibrating, and operating the PBS 0.5 Mini Vessel with a 60 µm perfusion filter. It covers required materials, pump configuration, vessel preparation, tubing installation, medium exchange, perfusion initiation, and routine operation. Step-by-step procedures address feed and waste management, flow-rate verification, troubleshooting, and maintenance. The manual serves as a practical reference for implementing continuous medium exchange and perfusion workflows within the PBS-MINI platform while maintaining controlled, sterile operation.

This data sheet presents the PBS-MINI Vertical-Wheel® Bioreactor platform for small-scale cell culture process development. It describes the universal base unit, 0.1 L and 0.5 L single-use Vessels, agitation technology, operating ranges, construction, and working volumes. The platform provides low-shear mixing, homogeneous particle suspension, scalability to larger PBS Bioreactors, and support for microcarrier and aggregate cultures. Technical specifications covering dimensions, agitation control, environmental requirements, materials, sterility, and regulatory compliance support platform…

This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…

This publication spotlight summarizes an independent study of human induced pluripotent stem cell expansion and differentiation into stem cell-derived islets using Vertical-Wheel® Bioreactors. A 27-day, six-stage suspension process was performed in 0.1 L and 0.5 L vessels. A fivefold increase in vessel scale generated a reported twelvefold increase in islet-equivalent yield while maintaining controlled islet size and limiting large aggregate formation. The resulting islets expressed human islet hormones and markers associated with beta-like cell identity, glucose responsiveness, and…

This application note evaluates human T-cell expansion in a PBS-MINI Vertical-Wheel® Bioreactor using CellGenix® GMP serum-free, xeno-free medium supplemented with IL-7 and IL-15. Studies compared cell growth, viability, phenotype, and expansion performance across multiple agitation rates and seeding densities relative to G-Rex culture systems. Lower agitation rates produced the highest viable cell yields while maintaining viabilities above 85%, whereas excessive agitation reduced expansion performance. Expanded cells achieved substantial fold expansion, high cell densities, favorable CD4+…

This application note compares human induced pluripotent stem cell expansion in a PBS-0.1 Mini Vertical-Wheel® Bioreator and a conventional horizontal-blade bioreactor. Single-cell-inoculated hiPSCs were cultured for six days in batch mode at 40, 60, and 80 rpm without medium exchange. The study evaluated viable cell expansion, aggregate size, and aggregate-size distribution across agitation rates. Vertical-Wheel® cultures achieved up to 16.7-fold expansion compared with 6.3-fold expansion in the horizontal-blade system. Aggregates in the Vertical-Wheel® Bioreactor remained below 300 µm and…

This application note evaluates linear scale-up of human induced pluripotent stem cell aggregate expansion from 0.1 to 3 L in PBS Vertical-Wheel® Bioreactors. TC1133 and PLX1 cell lines were inoculated as single cells and cultured using scale-correlated agitation, bolus feeding, and perfusion. Both scales demonstrated predictable growth, comparable cell concentrations, nearly identical specific growth rates, and consistent glucose and lactate profiles. Aggregates formed by day 1 and maintained healthy morphology through day 5. PBS-3 cultures retained high expression of SSEA-4, TRA-1-60…

This brochure introduces the PBS-0.5 Vertical-Wheel® PSC Perfusion Vessel, an integrated perfusion solution for pluripotent stem cell aggregate cultures and other large-particle processes. Continuous medium exchange through an integrated retention filter can support aggregate homogeneity, reduce manual handling, and enable downstream differentiation workflows. Comparative data show tighter aggregate-size distributions and smaller average aggregate diameters with perfusion than settlement-based medium exchange. The brochure also covers scalability, equipment compatibility, ordering…

This video demonstrates how to batch a PBS Mini 0.5 before inoculation. Batching adds medium and process components, excluding cells, so the vessel can equilibrate to the required temperature, pH, and dissolved oxygen in the incubator. The workflow covers aseptic unpacking, vessel inspection, working-volume planning, and liquid addition by pipetting or sterile pouring. These practices support a stable culture environment and prepare teams for similar setup steps at larger scales. The example reserves volume for later supplement and cell additions.

This video demonstrates inoculation after a PBS Mini vessel has equilibrated in the incubator. The example uses a 500 mL final working volume prepared with 400 mL of medium, leaving capacity for 50 mL of supplement and 50 mL of cells. The vessel remains on the base for active mixing while supplements and cells are added. Dispensing against the wall or directly into the liquid helps limit foaming and supports gentle cell transfer before the vessel returns to the incubator. The magnetic drive automatically couples when the vessel is placed on the base.

This video explains how to collect a representative sample from a PBS Mini culture for offline analysis, including cell counts and imaging. It covers sample-volume and frequency considerations, dedicated base use in a biosafety cabinet, and temporary agitation adjustments to eliminate unmixed regions before sampling. Viewers also learn the recommended pipette position and why the target volume should be collected in one continuous pull to improve sample consistency and data quality. The selected sampling speed is specific to the culture and vessel setup.

This video demonstrates an in-vessel, settle-based medium exchange for aggregate- or microcarrier-based cultures in the PBS Mini. It reviews when medium exchange may be appropriate and how culture type, particle size, culture stage, and growth kinetics influence settling. The workflow follows three steps: settle, remove, and add. A 50% exchange example shows how to aspirate clarified spent medium while limiting disturbance of retained cells and safely working around the Vertical-Wheel® impeller.

This video demonstrates two methods for completely harvesting cultures from a PBS-Mini Vertical-Wheel® Vessel. Operators can aspirate through the top opening using smaller-gauge serological pipettes that pass between the impeller blades to reach the vessel bottom. Alternatively, PBS-Mini 0.1 and PBS-Mini 0.5 Vessel configurations are available with a bottom port and connected tubing for streamlined bottom-up removal. Both methods support complete culture recovery based on the selected vessel configuration without requiring vessel disassembly.

This video introduces the perfusion-capable Vertical-Wheel FLUIDIX™ 0.5 Vessel, a ready-to-use, gamma-sterilized single-use product compatible with the universal PBS MINI base. The cap includes venting, a fresh-medium feed line, and a waste line with a 60-micron dip-tube filter that retains cell aggregates during medium removal. The video also reviews connection and pump-tubing options, line priming, opposing feed and waste flows, and pump programming to maintain a consistent working volume during perfusion.

This video introduces the PBS Mini single-use bioreactor system for small-scale cell culture development. It reviews the PBS Base and Vertical-Wheel FLUIDIX™ 0.1 and 0.5 Vessels, pre-gamma-sterilized double-bag packaging, and the universal base. Viewers learn how the U-shaped vessel and Vertical-Wheel® impeller support gentle mixing, how vessels connect through the dovetail mount, and how to operate the speed control, RPM display, and illumination features. The adjustable base operates from 5 to 100 RPM.

This user manual describes installation, configuration, operation, and maintenance of the PBS-MiniPRO Bioreactor System. It covers vessel setup, software operation, pH and dissolved oxygen control, perfusion workflows, medium exchange, sampling, harvesting, alarm management, reporting, batch management, automation, and calibration. The manual explains how PBS-MiniPRO combines small-scale process development with monitoring and control capabilities representative of larger PBS Bioreactors. It serves as an operational reference for implementing controlled, scalable cell culture processes.

The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.

This brochure introduces PBS MiniPRO as an automated, small-scale Vertical-Wheel® Bioreactor system for cell therapy process development. The platform provides dissolved oxygen and pH control, closed-system operation, perfusion-capable workflows, and parallel operation. As a scale-down model of PBS manufacturing-scale Bioreactors, MiniPRO supports predictive process development and technology transfer. The brochure highlights improved process control, reduced variability, streamlined scale-up, and support for workflows requiring consistent aggregate formation and gentle mixing conditions.

This user manual provides instructions for installing, configuring, operating, maintaining, calibrating, and troubleshooting the PBS-3 Bioreactor System with reusable pH and dissolved oxygen sensors. It covers agitation, temperature, gas control, pH, dissolved oxygen, sampling, and medium exchange. The manual also describes software functionality, user management, alarm configuration, recipe execution, reporting, data management, and system administration. It supports routine operation and validation of the PBS-3 platform in research, process development, and manufacturing environments.

This user manual describes the PBS-3 SUS Bioreactor System configured with single-use sensors. It provides instructions for installation, Vessel setup, calibration, operation, monitoring, sampling, harvesting, medium exchange, reporting, and system administration. The manual covers process-control functions, software operation, alarm management, and maintenance. It also explains the integrated single-use sensors and disposable Vessel assemblies designed to simplify operation while maintaining process control. The document serves as an operational reference for laboratory and manufacturing…

This user manual provides operational guidance for the updated PBS-3 Bioreactor System with reusable process sensors. It covers system setup, process control, software configuration, user administration, sampling, medium exchange, reporting, recipe and sequence execution, networking, and advanced system features. The manual includes descriptions of hardware components, control strategies, calibration methods, alarms, and maintenance procedures. It supports safe, standardized operation of the PBS-3 platform and reproducible process execution across process development and manufacturing…

This user manual covers configuration, operation, and maintenance of the updated PBS-3 SUS Bioreactor System with single-use sensors. It provides instructions for Vessel installation, startup, calibration, process control, sampling, harvesting, reporting, alarm management, user administration, and software operation. The manual also addresses automation, sequence execution, network integration, and data management. It supports standardized implementation of the PBS-3 SUS platform in environments requiring single-use process monitoring and scalable cell culture operation.

This data sheet summarizes the design, performance features, and technical specifications of the PBS-3 Vertical-Wheel® single-use Bioreactor System. It highlights low-shear mixing, homogeneous suspension, process scalability, integrated controls, and support for cell therapy manufacturing workflows. Specifications cover agitation, gas control, temperature management, sensors, Vessel construction, pumps, operating volumes, and communication capabilities. The document supports evaluation of PBS-3 for process development, scale-up studies, and early clinical manufacturing applications requiring…

This protocol describes a scalable suspension-bioprocess workflow for generating three-dimensional pancreatic islet organoids from human pluripotent stem cells. The process produces approximately 10,000 to 100,000 organoids that mature over four to five weeks into ?-like and ?-like endocrine cells capable of glucose-responsive insulin and glucagon secretion. The protocol details hPSC adaptation to suspension culture, passaging, cryopreservation, media preparation, and a six-stage directed differentiation process. Quality-control methods include morphology assessment, flow cytometry, cell…

This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…

This application note evaluates scalable CAR-NK cell expansion in PBS Vertical-Wheel® Bioreactors integrated with XCell® alternating tangential flow (ATF) perfusion. Human peripheral blood NK cells were activated, transduced, and expanded in PBS-3 bioreactors using batch, fed-batch, or perfusion feeding strategies. Studies assessed cell growth, metabolism, apoptosis, CAR expression, harvest, cryopreservation, and cytotoxic potency. A responsive perfusion strategy based on cell density and glucose consumption improved expansion compared with fixed perfusion schedules while maintaining…

This application note provides instructions for implementing perfusion medium exchange in a PBS-3 Vertical-Wheel® Bioreactor using a 60 µm polyethylene retention filter. Intended for aggregate and microcarrier-based pluripotent stem cell cultures, the document details system components, tubing assemblies, dip-tube placement, reservoir configuration, pump setup, and medium-exchange workflows. Guidance is provided for installation, sterilization, calibration, line priming, and filter disposal. Recommended flow rates are included for 0.5, 1, and 2 vessel volumes per day at a 3 L working volume.…

This application note evaluates linear scale-up of human induced pluripotent stem cell aggregate expansion from 0.1 to 3 L in PBS Vertical-Wheel® Bioreactors. TC1133 and PLX1 cell lines were inoculated as single cells and cultured using scale-correlated agitation, bolus feeding, and perfusion. Both scales demonstrated predictable growth, comparable cell concentrations, nearly identical specific growth rates, and consistent glucose and lactate profiles. Aggregates formed by day 1 and maintained healthy morphology through day 5. PBS-3 cultures retained high expression of SSEA-4, TRA-1-60…

The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.

This video introduces the next-generation PBS-3 Vertical-Wheel Vessel. The updated design retains the original vessel form, bioreactor fit, and Vertical-Wheel® geometry, maintaining the established hydrodynamic configuration. Enhancements include added structural support, a mechanically secured lid, three open PG13.5 ports, relocated headspace ports, and additional submerged lines. The expanded port layout provides greater flexibility for sensors, medium exchange, sampling, aspiration, harvest, and sterile liquid handling.

This user manual provides instructions for installing, configuring, operating, maintaining, and troubleshooting the PBS-15 SUS Bioreactor platform with single-use sensors. It covers Vessel installation, startup, process controls, gas management, calibration, sampling, harvesting, reporting, recipe execution, data management, and user administration. The manual also describes the software environment, alarm structures, and control-system functions required for controlled cell culture operation. It serves as a comprehensive reference for PBS-15 SUS users in process development and…

This user manual describes operation of the updated PBS-15 SUS Bioreactor configuration with single-use sensors. It covers installation, calibration, process control, Vessel handling, automation, reporting, user administration, network connectivity, and maintenance. Detailed procedures address media handling, sampling, harvesting, alarm management, and software configuration. The manual supports standardized operation and reproducible process execution while leveraging the scalability and control capabilities of the PBS-15 Vertical-Wheel® Bioreactor platform.

This user manual provides operating instructions for PBS-15 and PBS-80 Bioreactor systems configured with reusable pH and dissolved oxygen sensors. It covers installation, process setup, Vessel loading, calibration, automation, reporting, user management, software operation, maintenance, and troubleshooting. The manual describes control strategies for agitation, temperature, dissolved oxygen, pH, pressure, and fluid management across both systems. It serves as a reference for process development, technology transfer, manufacturing, and routine operation of large-scale Vertical-Wheel®…

This data sheet presents the PBS-15 Vertical-Wheel® single-use Bioreactor System and summarizes its features, specifications, and performance characteristics. The platform provides low-shear mixing, scalable hydrodynamics, integrated process controls, customizable fluid handling, and support for cell therapy manufacturing. Technical information covers vessel volume, dimensions, agitation, gas control, pumps, sensors, software, communication capabilities, and bag construction. The data sheet supports evaluation of PBS-15 for process development, scale-up, and clinical manufacturing workflows.

This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…

The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.

This user manual provides operating instructions for PBS-15 and PBS-80 Bioreactor systems configured with reusable pH and dissolved oxygen sensors. It covers installation, process setup, Vessel loading, calibration, automation, reporting, user management, software operation, maintenance, and troubleshooting. The manual describes control strategies for agitation, temperature, dissolved oxygen, pH, pressure, and fluid management across both systems. It serves as a reference for process development, technology transfer, manufacturing, and routine operation of large-scale Vertical-Wheel®…

This user manual provides instructions for setting up, operating, calibrating, maintaining, and administering the PBS-80 Bioreactor System with reusable process sensors. It covers system architecture, process controls, vessel installation, gas management, sampling, harvesting, reporting, alarm management, sequence execution, data management, and network integration. Operational procedures and technical reference information support reliable large-scale culture processes. The manual is intended for PBS-80 users in advanced process development, clinical production, and commercial manufacturing…

This data sheet provides an overview of the PBS-80 Vertical-Wheel® single-use Bioreactor System for large-scale cell therapy manufacturing. It highlights homogeneous mixing, low-shear suspension, integrated controls, scalable process performance, and customizable single-use fluid paths. Technical specifications cover working volume, agitation, gas delivery, process sensors, pumps, software, control architecture, and bag construction. The document supports evaluation of PBS-80 for clinical and commercial manufacturing applications requiring controlled, scalable, and reproducible bioprocess…

This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…

The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.