Xeno-Free T Cell Expansion in PBS Mini Vertical-Wheel® Bioreactor Using CellGenix® Reagents
This application note evaluates human T-cell expansion in a PBS-MINI Vertical-Wheel® Bioreactor using CellGenix® GMP serum-free, xeno-free medium supplemented with IL-7 and IL-15. Studies compared cell growth, viability, phenotype, and expansion performance across multiple agitation rates and seeding densities relative to G-Rex culture systems. Lower agitation rates produced the highest viable cell yields while maintaining viabilities above 85%, whereas excessive agitation reduced expansion performance. Expanded cells achieved substantial fold expansion, high cell densities, favorable CD4+ and CD8+ central memory phenotypes, and low exhaustion-marker expression. The document also highlights automatic magnetic activation-bead immobilization by the PBS magnetic-drive system, supporting streamlined and scalable T-cell manufacturing workflows.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
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