The Use of Vertical-Wheel® Technology for Superior Culture Homogeneity and Expansion of hiPSCs
This application note compares human induced pluripotent stem cell expansion in a PBS-0.1 Mini Vertical-Wheel® Bioreator and a conventional horizontal-blade bioreactor. Single-cell-inoculated hiPSCs were cultured for six days in batch mode at 40, 60, and 80 rpm without medium exchange. The study evaluated viable cell expansion, aggregate size, and aggregate-size distribution across agitation rates. Vertical-Wheel® cultures achieved up to 16.7-fold expansion compared with 6.3-fold expansion in the horizontal-blade system. Aggregates in the Vertical-Wheel® Bioreactor remained below 300 µm and maintained narrow, consistent size distributions, whereas horizontal-blade cultures produced larger, more heterogeneous aggregates exceeding 400 µm. The findings demonstrate improved culture homogeneity and aggregate expansion using Vertical-Wheel® Technology for scalable hiPSC manufacturing.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
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