Pluripotent Stem Cells
Scale PSC expansion and differentiation from 60mL to 80L on the Vertical-Wheel® Technology platform with precise control over aggregate culture growth and predictable process transfer to larger scales.
Designed for Expansion and Differentiation of PSCs
Control over aggregate size and hydrodynamic consistency are critical to reproducible PSC expansion and differentiation. Vertical-Wheel® Bioreactors provide homogeneous, low-shear mixing that helps maintain controlled aggregate size as processes move from development through scale-up.
Homogeneous mixing helps minimize uncontrolled aggregate merging during expansion, maintaining narrower size distributions that support more reproducible downstream differentiation.
Maintain a low-shear, low-EDR hydrodynamic environment from 60mL to 80L. CFD-characterized scale-up supports predictable process transfer from development-scale systems to larger working volumes.
Enable in-vessel differentiation through controlled aggregate sizing, supporting more consistent downstream outcomes from PSC expansion into SC-islets, organoids, and other lineages—all within the same vessel.
Proven Across PSC Workflows
Explore workflows supported by published studies, protocols, and application data across PSC expansion, aggregate and microcarrier culture, and directed differentiation.
Expert application support- PSC expansion
- Aggregate culture
- iPSC expansion
- Microcarrier culture
- 200 Billion iPSC at 50L
- Directed differentiation
- ESC expansion
- SC-islets
- Organoids
- High-density cell banking
Data that Supports Your Process
Explore peer-reviewed publications, protocols, posters, and technical resources demonstrating Vertical-Wheel® performance across PSC expansion, aggregate culture, scale-up, and differentiation workflows.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
This publication investigates hydrodynamic scale-up of human pluripotent stem cell aggregate cultures in 100 mL and 500 mL Vertical-Wheel® Bioreactors. Three-dimensional flow simulations and aggregate trajectory analysis were used to evaluate shear stress and energy dissipation rate exposure across multiple agitation conditions. Exposure profiles were compared with aggregation efficiency, cell growth, aggregate size, and fold expansion during six-day cultures. Energy dissipation rate exposure correlated with aggregate formation and expansion performance, while shear stress was not a…
This publication describes scalable generation of multilineage liver organoids from human pluripotent stem cells in a PBS-Mini Vertical-Wheel® Bioreactor. Studies evaluated how Activin A concentration directs endoderm and mesenchymal lineage development. High concentrations promoted homogeneous definitive endoderm induction and hepatic maturation markers, while lower concentrations generated early liver diverticula-like structures with lumens, hepatic mesenchyme, endothelial markers, and extracellular matrix organization. Transcriptomic, immunohistochemical, and developmental analyses…
This publication describes scalable human pluripotent stem cell expansion and growth factor-directed cardiomyocyte differentiation in Vertical-Wheel® Bioreactors. Studies evaluated inoculation, agitation, medium exchange, aggregate size, metabolism, viability, and pluripotency before optimizing differentiation with BMP4, Activin A, and bFGF. The process produced more than one million cardiac troponin T-positive cells/mL and was reproduced at 500 mL, averaging 1.11 million cardiomyocytes/mL and 93% cardiac purity. Cells displayed expected electrophysiological and contractile properties and…
This publication spotlight summarizes an independent study of human induced pluripotent stem cell expansion and differentiation into stem cell-derived islets using Vertical-Wheel® Bioreactors. A 27-day, six-stage suspension process was performed in 0.1 L and 0.5 L vessels. A fivefold increase in vessel scale generated a reported twelvefold increase in islet-equivalent yield while maintaining controlled islet size and limiting large aggregate formation. The resulting islets expressed human islet hormones and markers associated with beta-like cell identity, glucose responsiveness, and…
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