Use of 60 Micron Plastic (PE) Retention Filter for Perfusion MX with PBS-3 Vertical-Wheel® Bioreactor
This application note provides instructions for implementing perfusion medium exchange in a PBS-3 Vertical-Wheel® Bioreactor using a 60 µm polyethylene retention filter. Intended for aggregate and microcarrier-based pluripotent stem cell cultures, the document details system components, tubing assemblies, dip-tube placement, reservoir configuration, pump setup, and medium-exchange workflows. Guidance is provided for installation, sterilization, calibration, line priming, and filter disposal. Recommended flow rates are included for 0.5, 1, and 2 vessel volumes per day at a 3 L working volume. The protocol supports implementation of perfusion and continuous medium-exchange workflows while maintaining controlled operation within PBS-3 culture processes.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
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