Batching in PBS-Mini
This video demonstrates how to batch a PBS Mini 0.5 before inoculation. Batching adds medium and process components, excluding cells, so the vessel can equilibrate to the required temperature, pH, and dissolved oxygen in the incubator. The workflow covers aseptic unpacking, vessel inspection, working-volume planning, and liquid addition by pipetting or sterile pouring. These practices support a stable culture environment and prepare teams for similar setup steps at larger scales. The example reserves volume for later supplement and cell additions.
This application note demonstrates the benefits of PBS Biotech’s PBS-Mini Vertical-Wheel® Bioreactor within a scalable workflow developed with Bio-Techne for hiPSC expansion and differentiation into functional pancreatic islet-like clusters. The bioreactor supported robust aggregate culture while maintaining more than 95% pluripotency during hiPSC expansion and supporting progression through key stages of pancreatic differentiation. Combined with Bio-Techne’s GMP-grade reagents and analytical solutions, the workflow generated beta cell-containing, functional islet-like clusters that…
This webinar examines scalable suspension culture of PSC-derived organoids using PBS Biotech Vertical-Wheel bioreactors. PBS Applications Engineer, Hannah Worden, explains how low-shear, homogeneous mixing and consistent geometry support organoid process development and scale-up. Case studies cover liver, pancreatic islet, and cerebellar organoids, including aggregate-size control, differentiation, functional markers, and improved organization under dynamic culture. With the audience, we address agitation, seeding density, media formulation, perfusion-based medium exchange, sampling, and…
This poster, presented at ISSCR Kyoto 2026, demonstrates a scalable strategy for iPSC manufacturing using Vertical-Wheel Bioreactors, addressing expansion, process development, cell banking, and differentiation. Linear scale-up from PBS-0.1 through PBS-80 produced 200 billion iPSCs at a 50 L working volume while maintaining pluripotency and normal karyotype. High-density work cell banks were developed through 3D adaptation and retained differentiation potential toward cardiomyocytes and pancreatic progenitors. MiniPRO further served as a controlled scale-down model, demonstrating growth…
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