Publications Guide

This application note demonstrates the benefits of PBS Biotech’s PBS-Mini Vertical-Wheel® Bioreactor within a scalable workflow developed with Bio-Techne for hiPSC expansion and differentiation into functional pancreatic islet-like clusters. The bioreactor supported robust aggregate culture while maintaining  more than 95% pluripotency during hiPSC expansion and supporting progression through key stages of pancreatic differentiation. Combined with Bio-Techne’s GMP-grade reagents and analytical solutions, the workflow generated beta cell-containing, functional islet-like clusters that…

This webinar examines scalable suspension culture of PSC-derived organoids using PBS Biotech Vertical-Wheel bioreactors. PBS Applications Engineer, Hannah Worden, explains how low-shear, homogeneous mixing and consistent geometry support organoid process development and scale-up. Case studies cover liver, pancreatic islet, and cerebellar organoids, including aggregate-size control, differentiation, functional markers, and improved organization under dynamic culture. With the audience, we address agitation, seeding density, media formulation, perfusion-based medium exchange, sampling, and…

This presentation, presented at ISSCR Kyoto 2026 explores how Vertical-Wheel Bioreactors can reduce development and manufacturing risk for PSC therapies by providing a scalable platform from process development through commercial production. It highlights global clinical trials supported by Vertical-Wheel technology, predictive scale-down with MiniPRO, controlled hydrodynamics, automation, and linear scale-up through PBS-80. Data demonstrate consistent PSC growth across scales, culminating in greater than 200 billion cells from a 50 L batch while maintaining pluripotency, illustrating a…