Mesenchymal Stem/Stromal Cells
Scale MSC expansion and MSC-derived EV workflows from 60mL to 80L with homogeneous, low-shear mixing across microcarrier and aggregate cultures.
Designed for Scalable MSC Processing
Scaling MSC manufacturing requires efficient suspension, gentle mixing, and a consistent culture environment as volume increases. Vertical-Wheel® Bioreactors provide homogeneous, low-shear mixing to support MSC expansion across microcarrier and aggregate-based workflows.
Homogeneous, low-shear mixing keeps microcarriers and aggregates suspended while supporting MSC viability and phenotype throughout expansion.
Maintains even microcarrier suspension throughout culture, supporting consistent cell attachment, expansion, dissociation, and process performance.
Xeno-free fed-batch processes improve final cell yields and EV cargo across 60mL to 80L scales.
From MSC Expansion to EV Production
Explore MSC workflows supported by published studies, protocols, and application data across multiple tissue sources, culture formats, and process strategies.
Expert application support- BM-MSC expansion
- Cord-blood MSC
- Adipose-derived MSC
- Microcarrier culture
- Fed-batch processes
- MSC-derived EVs
Data That Supports Your Process
Explore published studies, protocols, and application data demonstrating MSC expansion, microcarrier processing, scale-up, and MSC-derived EV workflows in Vertical-Wheel® Bioreactors.
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This publication investigates scalable extracellular vesicle production from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel® Bioreactors. Studies evaluated the effects of media and agitation on metabolism, EV biogenesis, yield, and molecular cargo. Dynamic aggregate culture increased EV biogenesis pathway expression and vesicle production relative to static culture. Resulting EVs displayed expected markers and microRNA profiles associated with regenerative and immunomodulatory functions and modulated inflammatory responses in…
This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…
This publication describes a scalable microcarrier-based process for equine cord blood-derived mesenchymal stromal cell expansion in Vertical-Wheel® Bioreactors. Studies evaluated microcarrier selection, attachment, expansion, harvest, donor variability, serial passaging, and batch and fed-batch strategies. Development progressed from microcarrier screening to bioreactor optimization. The process achieved high cell densities while maintaining expected phenotype and functionality. Reproducible performance across multiple donors and passages demonstrated process robustness under dynamic…
This publication evaluates expansion of induced pluripotent stem cell-derived mesenchymal stromal cells on engineered gelatin methacryloyl microcarriers in Vertical-Wheel® Bioreactors. The study demonstrates scale-up of adherent microcarrier-based culture while enabling direct visualization of cell growth and efficient harvest through microcarrier digestion. Expanded cells maintained viability, differentiation potential, and immunomodulatory characteristics following expansion. The platform was also used to generate osteogenic matrix products that were evaluated in regenerative medicine…
This publication examines how expansion of bone marrow-derived human mesenchymal stromal cells in Vertical-Wheel® Bioreactors influences extracellular vesicle (EV) production and composition. Cells were cultured on microcarriers under controlled hydrodynamic conditions and compared with static culture systems. Bioreactor culture increased EV secretion, enhanced expression of EV biogenesis markers, and altered EV-associated microRNA and protein cargo. Proteomic and molecular analyses identified changes in pathways related to metabolism, autophagy, oxidative stress, and cellular regulation.…
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