PBS-3 User Manual (Reusable Sensors, Updated Platform)
This user manual provides operational guidance for the updated PBS-3 Bioreactor System with reusable process sensors. It covers system setup, process control, software configuration, user administration, sampling, medium exchange, reporting, recipe and sequence execution, networking, and advanced system features. The manual includes descriptions of hardware components, control strategies, calibration methods, alarms, and maintenance procedures. It supports safe, standardized operation of the PBS-3 platform and reproducible process execution across process development and manufacturing workflows.
The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.
This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…
This publication combines oxygen-transfer rate measurements through surface aeration for Vertical-Wheel bioreactors with specific oxygen-uptake measurements from human induced pluripotent stem cell aggregate cultures. Oxygen mass-transfer coefficients (kLa) were measured under different agitation rates, working volumes and headspace gas-flow conditions in 3, 15 and 80 L systems. The specific oxygen-uptake rate of iPSC aggregates was measured in a 0.1 L bioreactor using a custom dissolved oxygen probe configuration. These datasets were combined to estimate the theoretical maximum cell…
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