Extracellular Vesicles & Exosomes

This publication investigates scalable extracellular vesicle production from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel® Bioreactors. Studies evaluated the effects of media and agitation on metabolism, EV biogenesis, yield, and molecular cargo. Dynamic aggregate culture increased EV biogenesis pathway expression and vesicle production relative to static culture. Resulting EVs displayed expected markers and microRNA profiles associated with regenerative and immunomodulatory functions and modulated inflammatory responses in…

This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…

This publication evaluates extracellular vesicles (EVs) derived from interleukin-15-stimulated natural killer cells expanded in a PBS Vertical-Wheel® Bioreactor against osimertinib-resistant EGFR-mutant lung cancer. NK-EVs contained characteristic EV markers and cytotoxic proteins, including perforin and granzyme B, and demonstrated activity in 2D and 3D cultures. Combined treatment with carboplatin enhanced antitumor activity and reduced tumor burden in xenograft models. The study examines apoptosis, inflammatory signaling, and immune checkpoint regulation while demonstrating the potential…

This publication characterizes extracellular vesicles (EVs), including exosome-enriched populations, produced by human induced pluripotent stem cell-derived retinal organoids cultured in a Vertical-Wheel® Bioreactor. EVs from retinal organoids were compared with those from human umbilical cord mesenchymal stem cells based on concentration, morphology, protein content, nanomechanical properties, markers, and proteomic composition. Retinal organoid-derived EVs showed distinct protein signatures associated with retinal development and function, with differences across organoid developmental…

This publication examines how expansion of bone marrow-derived human mesenchymal stromal cells in Vertical-Wheel® Bioreactors influences extracellular vesicle (EV) production and composition. Cells were cultured on microcarriers under controlled hydrodynamic conditions and compared with static culture systems. Bioreactor culture increased EV secretion, enhanced expression of EV biogenesis markers, and altered EV-associated microRNA and protein cargo. Proteomic and molecular analyses identified changes in pathways related to metabolism, autophagy, oxidative stress, and cellular regulation.…

This webinar explores strategies for accelerating development, manufacturing, and commercialization of mesenchymal stem/stromal cell (MSC) and MSC-derived extracellular vesicle products. Topics include scale-up planning, cost-of-goods considerations, quality systems, process development, standardized cell banking, media selection, and robust product characterization. The presentation discusses scalable bioprocess platforms, including Vertical-Wheel® Bioreactors, and provides examples of improved productivity, consistency, and manufacturing efficiency across development and production scales.…