Bioreactor User Guide
This user guide provides an overview of PBS Biotech's Vertical-Wheel® Bioreactor platform for cell therapy process development and manufacturing. It describes the system's low-shear hydrodynamics, homogeneous mixing, and scalable vessel design for expanding iPSCs, MSCs, CAR-T, NK, and other mammalian cell types. The guide covers temperature control, gas management, agitation, oxygen transfer, cell density performance, utility requirements, and extractables testing. It also includes troubleshooting information, product configurations for PBS-MINI, PBS-3, PBS-15, and PBS-80 systems, a hiPSC sampling and counting protocol, and guidance on cGMP, ISO 9001:2015, and FDA 21 CFR Part 11 compliance.
This application note demonstrates the benefits of PBS Biotech’s PBS-Mini Vertical-Wheel® Bioreactor within a scalable workflow developed with Bio-Techne for hiPSC expansion and differentiation into functional pancreatic islet-like clusters. The bioreactor supported robust aggregate culture while maintaining more than 95% pluripotency during hiPSC expansion and supporting progression through key stages of pancreatic differentiation. Combined with Bio-Techne’s GMP-grade reagents and analytical solutions, the workflow generated beta cell-containing, functional islet-like clusters that…
This webinar examines scalable suspension culture of PSC-derived organoids using PBS Biotech Vertical-Wheel bioreactors. PBS Applications Engineer, Hannah Worden, explains how low-shear, homogeneous mixing and consistent geometry support organoid process development and scale-up. Case studies cover liver, pancreatic islet, and cerebellar organoids, including aggregate-size control, differentiation, functional markers, and improved organization under dynamic culture. With the audience, we address agitation, seeding density, media formulation, perfusion-based medium exchange, sampling, and…
This presentation, presented at ISSCR Kyoto 2026 explores how Vertical-Wheel Bioreactors can reduce development and manufacturing risk for PSC therapies by providing a scalable platform from process development through commercial production. It highlights global clinical trials supported by Vertical-Wheel technology, predictive scale-down with MiniPRO, controlled hydrodynamics, automation, and linear scale-up through PBS-80. Data demonstrate consistent PSC growth across scales, culminating in greater than 200 billion cells from a 50 L batch while maintaining pluripotency, illustrating a…
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