Scalable CAR-NK Cell Expansion Using Vertical-Wheel Bioreactors with XCell ATF Perfusion
This application note evaluates scalable CAR-NK cell expansion in PBS Vertical-Wheel® Bioreactors integrated with Repligen's XCell® alternating tangential flow (ATF) perfusion. Human peripheral blood NK cells were activated, transduced, and expanded in PBS-3 bioreactors using batch, fed-batch, or perfusion feeding strategies. Studies assessed cell growth, metabolism, apoptosis, CAR expression, harvest, cryopreservation, and cytotoxic potency. A responsive perfusion strategy based on cell density and glucose consumption improved expansion compared with fixed perfusion schedules while maintaining comparable cell health. At 3 L scale, perfusion generated higher expansion than fed-batch culture, and PBS-3-expanded cells demonstrated greater relative potency than static G-Rex controls. The application note also outlines XCell® ATF integration and medium-exchange workflows for CAR-NK manufacturing.
This poster, presented at ISSCR Kyoto 2026, demonstrates a scalable strategy for iPSC manufacturing using Vertical-Wheel Bioreactors, addressing expansion, process development, cell banking, and differentiation. Linear scale-up from PBS-0.1 through PBS-80 produced 200 billion iPSCs at a 50 L working volume while maintaining pluripotency and normal karyotype. High-density work cell banks were developed through 3D adaptation and retained differentiation potential toward cardiomyocytes and pancreatic progenitors. MiniPRO further served as a controlled scale-down model, demonstrating growth…
This presentation, presented at ISSCR Kyoto 2026 explores how Vertical-Wheel Bioreactors can reduce development and manufacturing risk for PSC therapies by providing a scalable platform from process development through commercial production. It highlights global clinical trials supported by Vertical-Wheel technology, predictive scale-down with MiniPRO, controlled hydrodynamics, automation, and linear scale-up through PBS-80. Data demonstrate consistent PSC growth across scales, culminating in greater than 200 billion cells from a 50 L batch while maintaining pluripotency, illustrating a…
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
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