Bioreactor Systems

PBS-80

The commercial-scale manufacturing bioreactor for allogeneic cell therapy production at scale. built on the same Vertical-Wheel® Technology validated at every previous scale.

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At a Glance

Working volume45–80L

VesselVertical-Wheel FLUIDIX™ 80L Vessels

Vessel GradeGMP-Research

Vessel MaterialPolyvinylidene Fluoride (PVDF)

Impeller MaterialPolycarbonate

SensorspH, DO

PerfusionATF Compatible

Instrument ConnectivityOPC-UA

Software Compliance21 CFR Part 11

Hero Gallery _ PBS-80 system — IA-80-B-511 : PBS-80 Vertical-Wheel Bioreactor

Highlights

Why PBS-80

The PBS-80 extends proven processes to commercial production volumes while preserving the culture environment your cells have been thriving in. It is designed to help manufacturers increase output, support growing demand, and maintain process consistency across the product lifecycle.

Commercial-Scale

Manufacture large cell therapy batches at production-relevant volumes.

Automated

Standardize large-scale manufacturing with recipes, process control, alarms, and comprehensive data capture.

GMP-Ready

Designed for commercial manufacturing with single-use, cGMP-ready vessels and secure, audit-ready, 21 CFR Part 11-compliant software and IQ/OQ documentation.

High-Output

Support high-density, high-yield production using external perfusion systems and closed-system manufacturing workflows.

Vertical-Wheel 80L

The PBS-80 Vertical-Wheel FLUIDIX™ Vessel

The PBS-80 runs pre-sterilized, single-use Vertical-Wheel FLUIDIX™ 80L Vessels for commercial-scale manufacturing to meet the market demand of your therapy.

Pre-sterilized & single-use: no cleaning or re-validation between runs.

Consistent wheel geometry: the same mixing your cells will meet at every scale.

Closed system: reduces contamination risk across your workflow.

Single-use sensors: measure, track, and control pH and DO through integrated optical sensors.

Evidence

Proven With PBS-80

Published Vertical-Wheel data at this scale — protocols, app. notes, peer-reviewed papers, case studies, posters, webinars, etc.

22 documents

Browse the library

FeaturedPosteriPSC & PSCDevelopment of Scalable and Robust Manufacturing Processes for iPSC-Derived Therapies Using Vertical-Wheel Bioreactor PlatformPBS-MiniPBS-3PBS-MiniPROPBS-80

PosterOpen document →

This poster, presented at ISSCR Kyoto 2026, demonstrates a scalable strategy for iPSC manufacturing using Vertical-Wheel Bioreactors, addressing expansion, process development, cell banking, and differentiation. Linear scale-up from PBS-0.1 through PBS-80 produced 200 billion iPSCs at a 50 L working volume while maintaining pluripotency and normal karyotype. High-density work cell banks were developed through 3D adaptation and retained differentiation potential toward cardiomyocytes and pancreatic progenitors. MiniPRO further served as a controlled scale-down model, demonstrating growth…

FeaturedShowcaseiPSC & PSCReducing Development Risk For Stem Cell Manufacturing With Scalable Vertical-Wheel BioreactorsPBS-MiniPBS-3PBS-MiniPROPBS-80

ShowcaseOpen document →

This presentation, presented at ISSCR Kyoto 2026 explores how Vertical-Wheel Bioreactors can reduce development and manufacturing risk for PSC therapies by providing a scalable platform from process development through commercial production. It highlights global clinical trials supported by Vertical-Wheel technology, predictive scale-down with MiniPRO, controlled hydrodynamics, automation, and linear scale-up through PBS-80. Data demonstrate consistent PSC growth across scales, culminating in greater than 200 billion cells from a 50 L batch while maintaining pluripotency, illustrating a…

FeaturedManualMSCs · iPSC & PSC · NK & CAR-NK · T Cells · EVs & Exosomes · Organoids & IsletsPublications GuidePBS-MiniPBS-MiniPROPBS-3PBS-15

ManualOpen document →

This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.

PosteriPSC & PSCPerfusion Feed and External Oxygenation Utilized to Achieve Linear Scale-Up of iPSC Culture into an 80 L Vertical-Wheel BioreactorPBS-3PBS-15PBS-80PBS-Mini

PosterOpen document →

This poster describes linear scale-up of human induced pluripotent stem cell aggregate expansion across 0.1, 3, 15, and 80 L Vertical-Wheel bioreactors. A baseline small-scale process was used to establish operating conditions and assess reproducibility and robustness across media, operators, cell lines, and agitation rates. Perfusion-based media exchange was introduced at reactor scales of 3 L and above to address increasing settlement times and aggregate merging as vessel scale increased. Cell growth, aggregate size, metabolic consumption and production, and pluripotency markers were…

PosteriPSC & PSC · Organoids & IsletsDevelopment of Scalable, Versatile, and Cost-Effective Processes for iPSC-Derived Cell Therapy Manufacturing via Linear Scale-Up and High-Density Cell Banking ApproachesPBS-3PBS-15PBS-80PBS-Mini

PosterOpen document →

This poster presents a three-dimensional process for linear scale-up of human induced pluripotent stem cell expansion from 0.1 to 80 L in Vertical-Wheel® Bioreactors. Cultures maintained growth, aggregate sphericity, pluripotency, karyotype, and functional pluripotency across scales. Serial aggregate culture and scalable harvest, concentration, and cryopreservation generated high-density working cell banks that retained viability, growth, genomic stability, and differentiation competence after thaw. The banks supported shortened endocrine, islet-like, and cardiomyocyte workflows through…

White PaperiPSC & PSC · MSCsCost-Effective Scaling of Cell Therapies: Vertical-Wheel Bioreactors vs Traditional SystemsPBS-3PBS-15PBS-80

White PaperOpen document →

This white paper examines the economic and operational advantages of Vertical-Wheel® Bioreactors compared with planar culture and conventional stirred-tank systems for cell therapy manufacturing. Key cost drivers include expansion efficiency, labor, facility footprint, process scalability, batch reproducibility, and product quality. Published examples demonstrate increased cell yield, fewer processing steps, reduced labor, and improved facility utilization through single-use, closed-system operation. Low-shear, homogeneous hydrodynamics support consistent growth and critical quality…