PBS-3
The bridge to the clinic: this is the single-use, cGMP-ready Vertical-Wheel® system to prove a process at scale before advancing to manufacturing.
Why PBS-3
This system is the step where process development becomes manufacturing readiness. The PBS-3 is designed to help teams transfer their PBS-MINI or MiniPRO-proven process can be transferred, standardized, and operated in a manufacturing-relevant environment.
Carry optimized process conditions from benchtop development into a manufacturing-relevant 3L scale.
Standardize every run with automated recipes that control process parameters, timed actions, and sequence-based workflows.
Designed for commercial and clinical manufacturing with single-use, cGMP-ready vessels and audit-ready 21 CFR Part 11-compliant software and IQ/OQ documentation.
Optimize the internal 60µm perfusion filter, or connect an external ATF system, to support high-density, closed-system processing and intensified workflows.
The PBS-3 Vertical-Wheel FLUIDIX™ Vessel
The PBS-3 runs pre-sterilized, single-use Vertical-Wheel FLUIDIX™ 3L Vessels for process scale-up and clinical manufacturing — building on development work established at smaller scales.
Proven With PBS-3
Published Vertical-Wheel data at this scale — protocols, app. notes, peer-reviewed papers, case studies, posters, webinars, etc.
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
This poster reviews key process development considerations required to achieve robust, efficient, and scalable manufacturing of allogeneic cell therapies**.** The authors discuss major scale-up challenges including equipment selection, hydrodynamic control, oxygen transfer, medium exchange, and cell harvest operations. Case studies demonstrate how process optimization and Vertical-Wheel bioreactor technology can reduce seed train requirements, shorten manufacturing timelines, decrease facility footprint, and integrate closed-system operation. The work also presents methodologies for…
This poster evaluates the PBS MiniPRO as a controlled scale-down platform for human induced pluripotent stem cell process development. The system combines four 500 mL Vertical-Wheel vessels with online pH and dissolved oxygen monitoring, gas control, integrated retention filters, perfusion pumps, and closed fluid-handling connections. Experiments examined process reproducibility, direct thaw from high-density cell banks, seed train, commercial and modified media, settlement and perfusion feeding, oxygen control, aggregate morphology, and sampling methods. Controlling dissolved oxygen at 50%…
The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.
This poster presents a closed iPSC manufacturing workflow integrating carrier-free aggregate perfusion, in-vessel harvest, concentration, formulation, automated fill-finish, and cryopreservation. A 3 L Vertical-Wheel® Bioreactor was compared with a 0.1 L scale-down process. Both achieved greater than 54-fold expansion, greater than 97% viability, and aggregate diameters of approximately 530 µm. The 3 L process generated approximately 3.7 billion viable cells, with 100% recovery after harvest and 95% post-harvest viability. Post-thaw studies assessed morphology, growth, pluripotency, and…
This flyer describes the PBS Biotech CDMO Partner Program, which provides flexible access to PBS-3 and MiniPRO Vertical-Wheel® Bioreactor systems through lease-to-own, installment, and on-demand acquisition models. The program helps CDMOs, CMOs, and process-development organizations expand cell therapy capabilities while reducing upfront capital requirements. Participants receive training, onboarding, technical support, and commercial collaboration opportunities. Program structures, deployment options, partner tiers, and qualification pathways are designed to support manufacturing capacity…
PBS-3 Parts - (selected parts are added to the cart for quote requests)
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We Support You Every Step of the Way
Our application scientists help you get the most out of PBS-3, and help you plan the steps that come next.
Our application scientists optimize cell yield and quality on PBS-3 with you.
Equivalent set points from PBS-3 to PBS-15, CFD-validated at every step.
Guided-optimization packages that move you from R&D to clinical scale.
Go Vertical, Today
Tell us what you need and we’ll take it from there.