PBS-Mini
The quickest way to start developing a scalable cell therapy. PBS-Mini® delivers a gentle, low-shear Vertical-Wheel® performance in a compact format: a true scale-down model for larger volumes.
Why PBS-Mini
The PBS-Mini helps teams generate meaningful process data quickly, without over-investing in equipment or infrastructure. This system is the true introduction to scalable bioprocessing and manufacturing of therapeutic cells, giving researchers a path from first experiments to large-scale commercial operations.
A low-risk, bench top entry point to prove feasibility with a representative small-scale model of our larger systems.
Representative scale-down model of our larger models through our patented Vertical-Wheel technology.
Runs 0.1L, 0.5L, and 0.5L perfusion configurations to support a wide range of cell types, culture formats, and development workflows.
The PBS-Mini Vertical-Wheel FLUIDIX™ Vessel
The PBS-Mini runs pre-sterilized, single-use Vertical-Wheel FLUIDIX™ Vessels in 0.1 L and 0.5 L sizes, using the same wheel geometry your cells will meet at every larger scale.
Proven With the PBS-Mini
Published Vertical-Wheel data at this scale — protocols, app. notes, peer-reviewed papers, case studies, posters, webinars, etc.
This application note demonstrates the benefits of PBS Biotech’s PBS-Mini Vertical-Wheel® Bioreactor within a scalable workflow developed with Bio-Techne for hiPSC expansion and differentiation into functional pancreatic islet-like clusters. The bioreactor supported robust aggregate culture while maintaining more than 95% pluripotency during hiPSC expansion and supporting progression through key stages of pancreatic differentiation. Combined with Bio-Techne’s GMP-grade reagents and analytical solutions, the workflow generated beta cell-containing, functional islet-like clusters that…
This webinar examines scalable suspension culture of PSC-derived organoids using PBS Biotech Vertical-Wheel bioreactors. PBS Applications Engineer, Hannah Worden, explains how low-shear, homogeneous mixing and consistent geometry support organoid process development and scale-up. Case studies cover liver, pancreatic islet, and cerebellar organoids, including aggregate-size control, differentiation, functional markers, and improved organization under dynamic culture. With the audience, we address agitation, seeding density, media formulation, perfusion-based medium exchange, sampling, and…
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
PBS-Mini Parts - (selected parts are added to cart for purchase or quote request)
Loading part numbers…
We Support You Every Step of the Way
Our application scientists help you get the most out of PBS-Mini, and help you plan the steps that comes next.
Our application scientists optimize cell yield and quality on PBS-Mini with you.
Equivalent set points from PBS-Mini to MiniPRO, CFD-validated at every step.
Guided-optimization packages that move you from R&D to clinical scale.
Go Vertical, Today
Tell us what you need and we’ll take it from there.