PBS-Mini
The quickest way to start developing a scalable cell therapy. PBS-Mini® delivers a gentle, low-shear Vertical-Wheel® performance in a compact format: a true scale-down model for larger volumes.
Why PBS-Mini
The PBS-Mini helps teams generate meaningful process data quickly, without over-investing in equipment or infrastructure. This system is the true introduction to scalable bioprocessing and manufacturing of therapeutic cells, giving researchers a path from first experiments to large-scale commercial operations.
A low-risk, bench top entry point to prove feasibility with a representative small-scale model of our larger systems.
Representative scale-down model of our larger models through our patented Vertical-Wheel technology.
Runs 0.1L, 0.5L, and 0.5L perfusion configurations to support a wide range of cell types, culture formats, and development workflows.
The PBS-Mini Vertical-Wheel FLUIDIX™ Vessel
The PBS-Mini runs pre-sterilized, single-use Vertical-Wheel FLUIDIX™ Vessels in 0.1 L and 0.5 L sizes, using the same wheel geometry your cells will meet at every larger scale.
Proven With the PBS-Mini
Published Vertical-Wheel data at this scale — protocols, app. notes, peer-reviewed papers, case studies, posters, webinars, etc.
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This brochure introduces the PBS-0.5 Vertical-Wheel® PSC Perfusion Vessel, an integrated perfusion solution for pluripotent stem cell aggregate cultures and other large-particle processes. Continuous medium exchange through an integrated retention filter can support aggregate homogeneity, reduce manual handling, and enable downstream differentiation workflows. Comparative data show tighter aggregate-size distributions and smaller average aggregate diameters with perfusion than settlement-based medium exchange. The brochure also covers scalability, equipment compatibility, ordering…
This poster presents a closed iPSC manufacturing workflow integrating carrier-free aggregate perfusion, in-vessel harvest, concentration, formulation, automated fill-finish, and cryopreservation. A 3 L Vertical-Wheel® Bioreactor was compared with a 0.1 L scale-down process. Both achieved greater than 54-fold expansion, greater than 97% viability, and aggregate diameters of approximately 530 µm. The 3 L process generated approximately 3.7 billion viable cells, with 100% recovery after harvest and 95% post-harvest viability. Post-thaw studies assessed morphology, growth, pluripotency, and…
PBS-Mini Parts - (selected parts are added to cart for purchase or quote request)
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We Support You Every Step of the Way
Our application scientists help you get the most out of PBS-Mini, and help you plan the steps that comes next.
Our application scientists optimize cell yield and quality on PBS-Mini with you.
Equivalent set points from PBS-Mini to MiniPRO, CFD-validated at every step.
Guided-optimization packages that move you from R&D to clinical scale.
Go Vertical, Today
Tell us what you need and we’ll take it from there.