PBS-3 Vertical-Wheel Bioreactor

Recommended for working cell culture volumes ranging from 1.8-3L

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Accelerate Scale Up Success for Your Cell Therapy Development

Accelerate your scale up success with the PBS-3 Vertical-Wheel Bioreactor —engineered for high-performance cell culture at the 1.8–3 L working volume. Seamlessly transition from the PBS-Mini or MinPRO and accelerate your path to clinical and commercial success.

Key Advantages for Your Cell Therapy

  • Established:  Single-use vessels and validated software ready for clinical manufacturing, including first-in-human applications
  • Versatile:  Configurable with single-use or reusable DO and pH sensors to fit your workflow
  • Optimized: Intuitive touchscreen interface for real-time process customization, monitoring, and data reporting

“ … cells harvested from the PBS-3 demonstrate greater CAR-NK potency compared to a static G-Rex control
condition…”
-Excerpt from the GeneFab and Repligen Case Study-

PBS-3 Bioreactors vs. Stirred Tank Bioreactors

Feature Vertical-Wheel Bioreactor Stirred Tank Bioreactor
Mixing Mechanism Vertical impeller that generates a gentle, large-scale, upward-and-outward flow. Horizontal impeller that creates high-shear zones and turbulent eddies.
Shear Stress Very low. Designed to minimize hydrodynamic stress, making it ideal for shear-sensitive cells like stem cells and cell aggregates. High, especially near the impeller blades. Can be detrimental to fragile cells, although impeller designs have been optimized to reduce this.
Scalability Linear. The hydrodynamic conditions remain consistent as the volume increases, allowing for predictable scale-up. Complex. Scaling up requires careful optimization to maintain consistent conditions, as fluid dynamics change with volume.
Homogeneity Excellent. The vertical wheel creates a highly uniform environment with eliminating occurrences of high turbulence. Good. Requires careful design to ensure uniform mixing and prevent gradients, especially in large vessels.
Applications Primary: Shear-sensitive cell cultures (hiPSCs, MSCs, T-cells), viral vectors, and exosomes Primary: Large-scale fermentation (bacteria, yeast), and general mammalian cell culture (e.g., CHO cells for biologics).

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Vertical-Wheel Bioreactor
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Single-use bioreactor company of the year 2025

Product Parts and Technical Specifications

Part Numbers
Specifications
Product Resources
Product Type Product Name Product Number  
Bioreactors PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors, 120V, v4 SW IA-3-B-711 Request a quote
PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors, 240V, v4 SW, Metric IA-3-B-712 Request a quote
Single-Use Vessels PBS-3 Single-Use Sensors, Research-GMP Grade, 400mL Asp. Port, 20mL Syringe IA-3-D-714 Request a quote
PBS-3 Single-Use Sensors, Commercial-GMP Grade, 400mL Asp. Port, 20mL Syringe GA-3-D-714 Request a quote
Accessories 60 Micron Plastic (PE) Retention Filters (pack of 5) IC01036 Request a quote
PBS-3 Dip Tube Assembly IA-3-BA-007 Request a quote
PBS Autoclavable Pouch, 4" x 15" (pack of 2) IA-UNI-DA-003 Request a quote
PBS Single-Syringe Sampling Line, 20mL IA-UNI-DA-006 Request a quote
PBS Autoclavable Pouch, 8" x 18" (pack of 2) IA-UNI-DA-007 Request a quote
Reusable DO Sensor, 12mm OD x 220mm L with Right-Angle IC00468 Request a quote
Reusable pH Sensor, 12mm OD x 225mm L C00469 Request a quote
Software PBS-3 Software v4 - 21 CFR Part 11 cGMP, Upgrade IA-3-SW-001 Request a quote
Qualification Packages PBS-3 Vertical-Wheel Bioreactor IQ/OQ Protocols IA-3-BQ-004 Request a quote
PBS-3 Vertical-Wheel Bioreactor IQ/OQ Configuration Sheet IA-3-BQ-005 Request a quote
Working volume range: 1.8 – 3.0 L
Gas control Mass flow controllers for air, N2, O2, CO2
Vessel Construction Injection-molded polycarbonate vessel
Product contact materials Meet requirements for USP Class VI Testing for Plastics <88> and/or ISO 10993
Download Full  Technical Spec Sheet

Vertical-Wheel Bioreactor
Scaling Calculator

Vertical-Wheel Bioreactor
Support Portal

Vertical-Wheel Bioreactor
Product Guides and Manuals

Single-use bioreactor company of the year 2025