PBS-3 Vertical-Wheel Bioreactor

The PBS-3 Vertical-Wheel^®  Bioreactor is the new paradigm for high-performance manufacturing of cell therapies. Seamlessly transition from the PBS-Mini or MinPRO and continue your scale up journey to clinical and commercial success. Recommended for working cell culture volumes ranging from 1.8 to 3 liters.

GMP Ready: validated single-use vessels and software ready for first-in-human applications
Versatile: single-use or reusable DO and pH sensors and customizable process control
Intuitive: plug and play out of the box and real-time data monitoring and reporting

PBS-3 Bioreactors vs. Stirred Tank Bioreactors

Feature	Vertical-Wheel Bioreactor	Stirred Tank Bioreactor
Mixing Mechanism	Vertical impeller that generates a gentle, large-scale, upward-and-outward flow.	Horizontal impeller that creates high-shear zones and turbulent eddies.
Shear Stress	Very low. Designed to minimize hydrodynamic stress, making it ideal for shear-sensitive cells like stem cells and cell aggregates.	High, especially near the impeller blades. Can be detrimental to fragile cells, although impeller designs have been optimized to reduce this.
Scalability	Linear. The hydrodynamic conditions remain consistent as the volume increases, allowing for predictable scale-up.	Complex. Scaling up requires careful optimization to maintain consistent conditions, as fluid dynamics change with volume.
Homogeneity	Excellent. The vertical wheel creates a highly uniform environment with eliminating occurrences of high turbulence.	Good. Requires careful design to ensure uniform mixing and prevent gradients, especially in large vessels.
Applications	Primary: Shear-sensitive cell cultures (hiPSCs, MSCs, T-cells), viral vectors, and exosomes	Primary: Large-scale fermentation (bacteria, yeast), and general mammalian cell culture (e.g., CHO cells for biologics).

View Product Resources

Vertical-Wheel Bioreactor
Scaling Calculator

Vertical-Wheel Bioreactor
Support Portal

Vertical-Wheel Bioreactor
Product Guides and Manuals

Request a Quote

Single-use bioreactor company of the year 2025

Product Parts and Technical Specifications

Product Type	Product Name	Product Number
Bioreactors	PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors, 120V, v4 SW	IA-3-B-711	Request a quote
Bioreactors	PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors, 240V, v4 SW, Metric	IA-3-B-712	Request a quote
Single-Use Vessels	PBS-3 Single-Use Sensors, Research-GMP Grade, 400mL Asp. Port, 20mL Syringe	IA-3-D-714	Request a quote
Single-Use Vessels	PBS-3 Single-Use Sensors, Commercial-GMP Grade, 400mL Asp. Port, 20mL Syringe	GA-3-D-714	Request a quote
Accessories	60 Micron Plastic (PE) Retention Filters (pack of 5)	IC01036	Request a quote
	PBS-3 Dip Tube Assembly	IA-3-BA-007	Request a quote
	PBS Autoclavable Pouch, 4" x 15" (pack of 2)	IA-UNI-DA-003	Request a quote
	PBS Single-Syringe Sampling Line, 20mL	IA-UNI-DA-006	Request a quote
	PBS Autoclavable Pouch, 8" x 18" (pack of 2)	IA-UNI-DA-007	Request a quote
	Reusable DO Sensor, 12mm OD x 220mm L with Right-Angle	IC00468	Request a quote
	Reusable pH Sensor, 12mm OD x 225mm L	C00469	Request a quote
Software	PBS-3 Software v4 - 21 CFR Part 11 cGMP, Upgrade	IA-3-SW-001	Request a quote
Qualification Packages	PBS-3 Vertical-Wheel Bioreactor IQ/OQ Protocols	IA-3-BQ-004	Request a quote
Qualification Packages	PBS-3 Vertical-Wheel Bioreactor IQ/OQ Configuration Sheet	IA-3-BQ-005	Request a quote

Working volume range:	1.8 – 3.0 L
Gas control	Mass flow controllers for air, N₂, O₂, CO₂
Vessel Construction	Injection-molded polycarbonate vessel
Product contact materials	Meet requirements for USP Class VI Testing for Plastics <88> and/or ISO 10993

Download Full Technical Spec Sheet

Vertical-Wheel Bioreactor
Scaling Calculator

Vertical-Wheel Bioreactor
Support Portal

Vertical-Wheel Bioreactor
Product Guides and Manuals