Tackling Allogeneic Immunotherapy’s Scale-up Bottleneck with the PBS Biotech Vertical-Wheel® Bioreactor
This white paper reviews how Vertical-Wheel® Bioreators can address manufacturing challenges associated with allogeneic NK-cell therapies. The document summarizes studies comparing NK-cell expansion and cytotoxic activity in Vertical-Wheel® Bioreators and G-Rex systems across multiple agitation rates and scale-up scenarios. Vertical-Wheel® cultures achieved higher expansion and cytotoxic performance while reducing the number of vessels required to reach manufacturing-scale targets. The paper also highlights research in which NK cells expanded in a Vertical-Wheel® Bioreator were used to produce extracellular vesicles for investigation in resistant lung cancer models. Together, these examples demonstrate how scalable bioreactor platforms can support development of allogeneic NK-cell and NK-EV-based immunotherapies.
This publication investigates scalable extracellular vesicle production from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel® Bioreactors. Studies evaluated the effects of media and agitation on metabolism, EV biogenesis, yield, and molecular cargo. Dynamic aggregate culture increased EV biogenesis pathway expression and vesicle production relative to static culture. Resulting EVs displayed expected markers and microRNA profiles associated with regenerative and immunomodulatory functions and modulated inflammatory responses in…
This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…
This publication evaluates extracellular vesicles (EVs) derived from interleukin-15-stimulated natural killer cells expanded in a PBS Vertical-Wheel® Bioreactor against osimertinib-resistant EGFR-mutant lung cancer. NK-EVs contained characteristic EV markers and cytotoxic proteins, including perforin and granzyme B, and demonstrated activity in 2D and 3D cultures. Combined treatment with carboplatin enhanced antitumor activity and reduced tumor burden in xenograft models. The study examines apoptosis, inflammatory signaling, and immune checkpoint regulation while demonstrating the potential…
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