Mesenchymal Stem/Stromal Cells
Scale MSC expansion and MSC-derived EV workflows from 60mL to 80L with homogeneous, low-shear mixing across microcarrier and aggregate cultures.
Designed for Scalable MSC Processing
Scaling MSC manufacturing requires efficient suspension, gentle mixing, and a consistent culture environment as volume increases. Vertical-Wheel® Bioreactors provide homogeneous, low-shear mixing to support MSC expansion across microcarrier and aggregate-based workflows.
Homogeneous, low-shear mixing keeps microcarriers and aggregates suspended while supporting MSC viability and phenotype throughout expansion.
Maintains even microcarrier suspension throughout culture, supporting consistent cell attachment, expansion, dissociation, and process performance.
Xeno-free fed-batch processes improve final cell yields and EV cargo across 60mL to 80L scales.
From MSC Expansion to EV Production
Explore MSC workflows supported by published studies, protocols, and application data across multiple tissue sources, culture formats, and process strategies.
Expert application support- BM-MSC expansion
- Cord-blood MSC
- Adipose-derived MSC
- Microcarrier culture
- Fed-batch processes
- MSC-derived EVs
Data That Supports Your Process
Explore published studies, protocols, and application data demonstrating MSC expansion, microcarrier processing, scale-up, and MSC-derived EV workflows in Vertical-Wheel® Bioreactors.
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This user manual provides instructions for setting up, calibrating, and operating the PBS 0.5 Mini Vessel with a 60 µm perfusion filter. It covers required materials, pump configuration, vessel preparation, tubing installation, medium exchange, perfusion initiation, and routine operation. Step-by-step procedures address feed and waste management, flow-rate verification, troubleshooting, and maintenance. The manual serves as a practical reference for implementing continuous medium exchange and perfusion workflows within the PBS-MINI platform while maintaining controlled, sterile operation.
This publication investigates scalable extracellular vesicle production from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel® Bioreactors. Studies evaluated the effects of media and agitation on metabolism, EV biogenesis, yield, and molecular cargo. Dynamic aggregate culture increased EV biogenesis pathway expression and vesicle production relative to static culture. Resulting EVs displayed expected markers and microRNA profiles associated with regenerative and immunomodulatory functions and modulated inflammatory responses in…
This white paper examines the economic and operational advantages of Vertical-Wheel® Bioreactors compared with planar culture and conventional stirred-tank systems for cell therapy manufacturing. Key cost drivers include expansion efficiency, labor, facility footprint, process scalability, batch reproducibility, and product quality. Published examples demonstrate increased cell yield, fewer processing steps, reduced labor, and improved facility utilization through single-use, closed-system operation. Low-shear, homogeneous hydrodynamics support consistent growth and critical quality…
This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…
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