Extracellular Vesicles & Exosomes
Increase EV and exosome production through scalable 3D dynamic culture while supporting distinct cargo profiles across cell-derived vesicle workflows.
Move Beyond 2D EV Production
EV production depends not only on the number of producer cells, but also on the culture environment in which they are grown. Vertical-Wheel® Bioreactors enable 3D dynamic culture of aggregates and microcarriers, supporting increased EV secretion and a scalable path beyond planar culture.
3D aggregate and microcarrier culture under dynamic conditions can increase EV secretion compared with static planar culture.
Dynamic 3D culture can influence the proteomic and molecular cargo profile of secreted vesicles compared with planar culture.
Move from development-scale studies toward larger-volume EV production without relying on increasingly large planar culture footprints.
EV Workflows & Methodologies
Explore extracellular vesicle workflows spanning multiple producer cell types, 3D culture formats, and emerging therapeutic applications.
Expert Application Support- MSC-Derived EVs
- NK-Derived EVs
- Organoid-Derived exosomes
- EV Manufacturing
- Drug-Delivery vesicles
Data Behind EV Production
Explore publications, application data, and technical resources examining EV secretion, cargo profiles, and scalable production in Vertical-Wheel® Bioreactors.
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
This publication investigates scalable extracellular vesicle production from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel® Bioreactors. Studies evaluated the effects of media and agitation on metabolism, EV biogenesis, yield, and molecular cargo. Dynamic aggregate culture increased EV biogenesis pathway expression and vesicle production relative to static culture. Resulting EVs displayed expected markers and microRNA profiles associated with regenerative and immunomodulatory functions and modulated inflammatory responses in…
This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…
This white paper reviews how Vertical-Wheel® Bioreators can address manufacturing challenges associated with allogeneic NK-cell therapies. The document summarizes studies comparing NK-cell expansion and cytotoxic activity in Vertical-Wheel® Bioreators and G-Rex systems across multiple agitation rates and scale-up scenarios. Vertical-Wheel® cultures achieved higher expansion and cytotoxic performance while reducing the number of vessels required to reach manufacturing-scale targets. The paper also highlights research in which NK cells expanded in a Vertical-Wheel® Bioreator were used to…
This publication evaluates extracellular vesicles (EVs) derived from interleukin-15-stimulated natural killer cells expanded in a PBS Vertical-Wheel® Bioreactor against osimertinib-resistant EGFR-mutant lung cancer. NK-EVs contained characteristic EV markers and cytotoxic proteins, including perforin and granzyme B, and demonstrated activity in 2D and 3D cultures. Combined treatment with carboplatin enhanced antitumor activity and reduced tumor burden in xenograft models. The study examines apoptosis, inflammatory signaling, and immune checkpoint regulation while demonstrating the potential…
This publication characterizes extracellular vesicles (EVs), including exosome-enriched populations, produced by human induced pluripotent stem cell-derived retinal organoids cultured in a Vertical-Wheel® Bioreactor. EVs from retinal organoids were compared with those from human umbilical cord mesenchymal stem cells based on concentration, morphology, protein content, nanomechanical properties, markers, and proteomic composition. Retinal organoid-derived EVs showed distinct protein signatures associated with retinal development and function, with differences across organoid developmental…
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