Upscaling Human Mesenchymal Stromal Cell Production in a Novel Vertical-Wheel Bioreactor Enhances Extracellular Vesicle Secretion and Cargo Profile
This publication examines how expansion of bone marrow-derived human mesenchymal stromal cells in Vertical-Wheel® Bioreactors influences extracellular vesicle (EV) production and composition. Cells were cultured on microcarriers under controlled hydrodynamic conditions and compared with static culture systems. Bioreactor culture increased EV secretion, enhanced expression of EV biogenesis markers, and altered EV-associated microRNA and protein cargo. Proteomic and molecular analyses identified changes in pathways related to metabolism, autophagy, oxidative stress, and cellular regulation. Scale-up studies demonstrated consistent EV productivity across bioreactor volumes. The findings provide insight into how bioprocess conditions influence MSC-derived EV manufacturing and support development of scalable production strategies for cell-free therapeutics.
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This publication investigates scalable extracellular vesicle production from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel® Bioreactors. Studies evaluated the effects of media and agitation on metabolism, EV biogenesis, yield, and molecular cargo. Dynamic aggregate culture increased EV biogenesis pathway expression and vesicle production relative to static culture. Resulting EVs displayed expected markers and microRNA profiles associated with regenerative and immunomodulatory functions and modulated inflammatory responses in…
This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…
How Can We Help?
Tell us what you need and we’ll take it from there.