Scalable Manufacturing of Human Mesenchymal Stem/Stromal Cells and Induced Pluripotent Stem Cells in Vertical-Wheel Bioreactors
This poster summarizes manufacturing strategies for expansion of human mesenchymal stem/stromal cells and human induced pluripotent stem cells in Vertical-Wheel® Bioreactors. For MSC manufacturing, adherent microcarrier-based culture achieved high cell densities with efficient harvest across multiple scales. For iPSC expansion, computational fluid dynamics and energy dissipation rate analysis were used to establish relationships between agitation conditions and aggregate morphology. These engineering models guided scale-up and supported consistent culture performance between reactor sizes. The work demonstrates how quantitative engineering principles can support both adherent and aggregate-based manufacturing workflows while enabling reproducible process development and scalable cell production.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
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