PBS Closed-System Sampling Conical (50 mL)

This application note describes an hMSC manufacturing workflow using RoosterBio cell products, xeno-free media, and PBS Biotech Vertical-Wheel bioreactors. Studies evaluated microcarrier culture, fed-batch operation, harvest, scale-up, and cell quality. The low-shear, uniformly mixed environment supported robust attachment and cell densities up to 500,000 cells/mL within five days. Fed-batch processing improved media productivity by eliminating routine media exchanges and reducing labor. Comparable growth and metabolite control were maintained across development, pilot, and production…

This publication combines oxygen-transfer rate measurements through surface aeration for Vertical-Wheel bioreactors with specific oxygen-uptake measurements from human induced pluripotent stem cell aggregate cultures. Oxygen mass-transfer coefficients (kLa) were measured under different agitation rates, working volumes and headspace gas-flow conditions in 3, 15 and 80 L systems. The specific oxygen-uptake rate of iPSC aggregates was measured in a 0.1 L bioreactor using a custom dissolved oxygen probe configuration. These datasets were combined to estimate the theoretical maximum cell…

This video introduces the next-generation PBS-3 Vertical-Wheel Vessel. The updated design retains the original vessel form, bioreactor fit, and Vertical-Wheel® geometry, maintaining the established hydrodynamic configuration. Enhancements include added structural support, a mechanically secured lid, three open PG13.5 ports, relocated headspace ports, and additional submerged lines. The expanded port layout provides greater flexibility for sensors, medium exchange, sampling, aspiration, harvest, and sterile liquid handling.