Optimizing Closed-System iPSC Processes Using a Scaled-Down, Computer-Controlled Parallel Bioreactor Platform
This poster evaluates the PBS MiniPRO as a controlled scale-down model for iPSC development, compared with non-controlled PBS-0.5 L Mini Perfusion vessels. All PBS-0.5 L, MiniPRO, and PBS-3 L systems used perfusion culture. Maintaining 50% dissolved oxygen from inoculation increased yield potential and physicochemical stability by avoiding oxygen limitation and fluctuations. Results were consistent across 2D-to-3D and direct-thaw seed trains and mTeSR and modified B8 media. MiniPRO and PBS-3 L cultures achieved comparable growth, aggregate size, and metabolism while maintaining perfusion, 50% dissolved oxygen control, and volume-averaged energy dissipation rate. The findings demonstrate robust, linear scale-up and closed-system operation.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.
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