Scalable Manufacture of Therapeutic Mesenchymal Stromal Cell Products on Customizable Microcarriers in Vertical Wheel Bioreactors that Improve Direct Visualization, Product Harvest, and Cost
This publication evaluates expansion of induced pluripotent stem cell-derived mesenchymal stromal cells on engineered gelatin methacryloyl microcarriers in Vertical-Wheel® Bioreactors. The study demonstrates scale-up of adherent microcarrier-based culture while enabling direct visualization of cell growth and efficient harvest through microcarrier digestion. Expanded cells maintained viability, differentiation potential, and immunomodulatory characteristics following expansion. The platform was also used to generate osteogenic matrix products that were evaluated in regenerative medicine models. In addition to biological performance, the authors assessed manufacturing economics and operational efficiency relative to conventional monolayer culture. The findings support an integrated microcarrier and Vertical-Wheel® Bioreactor strategy for scalable production, monitoring, and harvest of therapeutic MSC-derived products.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
How Can We Help?
Tell us what you need and we’ll take it from there.