Production of Oncolytic Adenovirus and Human Mesenchymal Stem Cells in a Single-Use, Vertical-Wheel Bioreactor System: Impact of Bioreactor Design on Performance of Microcarrier-Based Cell Culture Processes
This publication presents engineering characterization of PBS Vertical-Wheel® Bioreactors using Power number versus Reynolds number analysis, a standard framework for evaluating mixing performance. A gravimetric method for measuring power draw was developed and validated against a baffled stirred-tank bioreactor equipped with a Rushton impeller before being applied to PBS 0.5 L, 3 L, and 15 L Vertical-Wheel® Bioreactors. The study identified laminar, transitional, and turbulent flow regimes and demonstrated consistent scaling behavior across vessel sizes. Low and comparable turbulent-regime power numbers supported geometric scaling consistency and provide data for bioreactor characterization, computational fluid dynamics validation, energy dissipation analysis, and process scale-up.
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in adherent culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems the PBS-Mini bioreactor. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell…
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
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