Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organoids reveals faster commitment under dynamic conditions
Introduction
Human‐induced pluripotent stem cells (iPSCs) have great potential for disease modeling. However, generating iPSC‐derived models to study brain diseases remains a challenge. In particular, the ability to recapitulate cerebellar development in vitro is still limited. We presented a reproducible and scalable production of cerebellar organoids by using the novel single‐use Vertical‐Wheel bioreactors, in which functional cerebellar neurons were obtained.
Topics covered in this note
- Generation, differentiation, and maturation of human iPSC‐derived aggregates using Vertical‐Wheel bioreactors
- Dynamic culture promotes a significant enrichment of extracellular matrix during cerebellar differentiation
- Dynamic condition allows a reproducible and large‐scale generation of organoids
