PBS-0.5 Mini Vertical-Wheel Bioreactor

Recommended for working cell culture volumes
ranging from 300-500mL

The Best Choice for Your Early Stage Cell Therapy Optimization

The PBS-0.5 Mini^® single use bioreactor supports working volumes from 300 to 500 mL , offering an ideal platform for early stage cell therapy optimization. Vertical-Wheel^® mixing technology ensures homogeneous conditions and linear scale up to larger systems.

Flexible platform: a universal base unit supports PBS-0.1, PBS-0.5, and PBS-0.5 Mini Perfusion single-use vessels, enabling smooth process scalability.
GMP readiness: all product-contact materials comply with regulatory standards for use in first-in-human clinical trials.
Sterile handling: removable vessel caps allow straightforward aseptic liquid transfer in a biosafety cabinet.

“PBS-Minis provide an easy-to-use, cost-effective scale-down model for early process development…Learnings from these studies were used to test process parameters which were successfully translated into the PBS-3 Vertical-Wheel Bioreactor.”
-Excerpt from GeneFab and Repligen Case Study-

PBS-0.5 Mini vs. Stirred Tank Bioreactors

Feature	Vertical-Wheel Bioreactor	Stirred Tank Bioreactor
Mixing Mechanism	Vertical-Wheel impeller that generates gentle and scalable fluid conditions.	Horizontal impeller that creates wide range of turbulent eddies and zones of highly variable flow.
Hydrodynamic Conditions	Homogeneous fluid flow throughout the vessel, meaning all cells experience similiar growth conditions.	Heterogeneous, with cells experiencing variable conditions depending on location within vessel.
Shear Stress	Consistently low throughout the environment, which is ideal for shear-sensitive cells such as those on microcarriers or grown as aggregates.	High, especially near tips of impeller blades, which can be detrimental to shear-sensitive cells.
Scalability	Linear, with reproducible hydrodynamic conditions at all volumes thus allowing for predictable scale-up.	Complex, with scale up requiring careful optimization to maintain consistent conditions.
Applications	Primarily larger particles (aggregates, microcarriers) of adherent, shear-sensitive cells (hiPSCs, MSCs, T-cells).	Primarily single cells for mammalian (CHO for biologics) or fermentation (bacterial, yeast) cultures.

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Product Parts and Technical Specifications

Product Type	Product	Product Number
Base Unit	PBS-Mini Vertical-Wheel Bioreactor Base Unit (120-240 V)	FA-UNI-B-501	Request a quote
Single-Use Vessels	PBS-0.5 Vertical-Wheel Single-Use Vessel with Bottom Port (pack of 1)	FA-0.5-D-101	Request a quote
	PBS-0.5 Vertical-Wheel Single-Use Vessel (pack of 4)	FA-0.5-D-001	Request a quote
	PBS 0.5 Single-Use Vessel with 60μm PSC Perfusion Filter (pack of 1)	IA-0.5-D-201	Request a quote
Accessories	PBS-Mini Extended 4-Gang DC Power Distribution Cable	IA-UNI-BA-112	Request a quote

Working volume range:	100 - 300 mL (For liquid mixing) 300 - 500 mL (For cell culture)
Number of ports	2
Gassing	Diffusion through 0.22 micron filter on vent cap
Liquid transfer	Port with screw-on vent cap
Product contact materials	Meet requirements for USP Class VI Testing for Plastics and/or ISO 10993

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