PBS-0.5 Mini Perfusion Single-Use Vessel

Recommended for working cell culture volumes ranging from 400-500mL

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Efficiently Scale Up Perfusion-based PSC Cultures

The PBS 0.5L Single-Use Vessel with PSC Perfusion Filter (60 µm) empowers researchers to scale up perfusion-based PSC cultures efficiently maintaining cell quality and process control from small-scale experiments to clinical production.

Key Advantages for Your Cell Therapy

  • Engineered for Controlled Perfusion: Achieve uniform PSC aggregates and superior differentiation results through consistent medium exchange, supporting high yields in regenerative medicine and cell therapy workflows
  • Integrated Sterile System: The built-in 60 µm cell retention filter with dedicated feed and waste lines ensures contamination-free, continuous perfusion—ideal for testing multiple feeding or media optimization strategies
  • Flexible and Pump-Ready: Designed with both standard and Masterflex® High-Performance Precision Pump silicone tubing for direct compatibility with leading peristaltic pumps. C-Flex® tubing supports sterile welding and extended line configurations for easy system integration
  • Seamless Bioreactor Compatibility: Pairs perfectly with the PBS-Mini Vertical-Wheel Bioreactor Base Unit (P/N: FA-UNI-B-501) to provide a compact, scalable solution for advanced perfusion research and process development

“ Perfusion culture yielded a 2.6-fold higher expansion than the fed-batch run from the same donor, highlighting its advantage in achieving substantially greater cell yields… These findings highlight the benefits of perfusion in supporting robust cell growth and promoting a more productive culture environment. ”
-Excerpt from the GeneFab and Repligen Case Study »

PBS Bioreactors vs. Stirred Tank Bioreactors

Feature Vertical-Wheel Bioreactor Stirred Tank Bioreactor
Mixing Mechanism Vertical impeller that generates a gentle, large-scale, upward-and-outward flow. Horizontal impeller that creates high-shear zones and turbulent eddies.
Shear Stress Very low. Designed to minimize hydrodynamic stress, making it ideal for shear-sensitive cells like stem cells and cell aggregates. High, especially near the impeller blades. Can be detrimental to fragile cells, although impeller designs have been optimized to reduce this.
Scalability Linear. The hydrodynamic conditions remain consistent as the volume increases, allowing for predictable scale-up. Complex. Scaling up requires careful optimization to maintain consistent conditions, as fluid dynamics change with volume.
Homogeneity Excellent. The vertical wheel creates a highly uniform environment with eliminating occurrences of high turbulence. Good. Requires careful design to ensure uniform mixing and prevent gradients, especially in large vessels.
Applications Primary: Shear-sensitive cell cultures (hiPSCs, MSCs, T-cells), viral vectors, and exosomes Primary: Large-scale fermentation (bacteria, yeast), and general mammalian cell culture (e.g., CHO cells for biologics).
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Product Parts and Technical Specifications

Part Numbers
Specifications
Product Resources
Product Type Product Product Number  
Base Unit PBS-Mini Vertical-Wheel Bioreactor Base Unit (120-240 V) FA-UNI-B-501 Request a quote
Single-Use Vessels PBS 0.5 Single-Use Vessel with 60μm PSC Perfusion Filter (pack of 1) IA-0.5-D-201 Request a quote
Accessories
60 Micron Plastic (PE), Retention filter (5-pack) IC01036 Request a quote
PBS-3 Dip Tube Assembly (pack of 1) IA-3-BA-007 Request a quote
Working volume range: 400-500mL (500mL recommended)
Number of ports 2
Gassing Diffusion through 0.22 micron filter on vent cap
Liquid transfer Port with screw-on vent cap
Product contact materials Meet requirements for USP Class VI Testing for Plastics and/or ISO 10993

Vertical-Wheel Bioreactor
Scaling Calculator

Vertical-Wheel Bioreactor
Support Portal

Vertical-Wheel Bioreactor
Product Guides and Manuals

Single-use bioreactor company of the year 2025

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If you are not satisfied with the performance of the PBS-Mini products or accessories get your money back.  Must show necessary performance documentation to qualify.