Expansion of MSCs on Microcarriers in the PBS-Mini Vertical-Wheel Bioreactor

This protocol details a scalable workflow for expanding mesenchymal stem/stromal cells (MSCs), beginning with 2D seed cultures in T-flasks and transitioning to dynamic 3D growth on the surface of suspended microcarriers in PBS-Mini Vertical-Wheel^® bioreactors. Designed for reproducibility and translational relevance, this framework enables precise control over attachment, expansion, detachment, and harvesting — providing a robust foundation for downstream applications in regenerative and cell-based therapies.