Bioprocess development for cord blood mesenchymal stromal cells on microcarriers in Vertical‐Wheel bioreactors
Introduction
Equine mesenchymal stromal cells (MSCs) have been found to be beneficial for the treatment of many ailments, including orthopedic injuries, due to their superior differentiation potential and immunomodulating properties. Cell therapies require large cell numbers, which are not efficiently generated using conventional static expansion methods. Expansion of equine cord blood‐derived MSCs (eCB‐MSCs) in bioreactors, using microcarriers as an attachment surface, has the potential to generate large numbers of cells with increased reproducibility and homogeneity compared with static T‐flask expansion.
Topics covered in this note
- Bioreactor culture of eCB‐MSCs
- Harvesting of eCB‐MSCs from microcarriers
- Microcarrier screening in bioreactors
